nested-PCR
常見例句
- Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
利用套式PCR技術(shù)檢測母血中胎兒SRY基因的研究 - Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR.
巢式PCR引所需要兩套用于放大特定的DNA片段,用兩條擠提聚合酶鏈。 - In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.
4 7份ELISA檢測HCV抗體陽性血清標本 ;RT nested PCR檢出陽性標本 33例 (70 .;2 1%25 );RT PCR DDH檢出陽性標本 39例 (82 - Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
獲取正常男性單個淋巴細胞和無DMD家族史的單個胚胎細胞,行DMD基因外顯子17、19、44、45、48的五重巢式PCR,分析擴增成功率、假陽性率和假陰性率。 - In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay.
在對2份PPD檢測陰性而巢式PCR檢測陽性的臨床樣本的檢測結(jié)果也為陽性。 - Two pairs of primers were dsigned from cholera toxin subunit A(ctx A)gene and a nested PCR that can detect O1 classical (CVC),El Tor (EVC)and O 139 especially was established.
針對霍亂弧菌腸毒素A亞單位(ctx?A)基因設(shè)計兩對引物,建立套式PCR,可特異擴增霍亂弧菌O1群古典型、埃爾托型和O139群; 返回 nested-PCR