nested-PCR

基本解釋

Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR.
利用套式PCR技術(shù)檢測(cè)母血中胎兒SRY基因的研究
Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR.
巢式PCR引所需要兩套用于放大特定的DNA片段,用兩條擠提聚合酶鏈。
In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.
4 7份ELISA檢測(cè)HCV抗體陽(yáng)性血清標(biāo)本 ;RT nested PCR檢出陽(yáng)性標(biāo)本 33例 (70 .;2 1%25 );RT PCR DDH檢出陽(yáng)性標(biāo)本 39例 (82
Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established.
獲取正常男性單個(gè)淋巴細(xì)胞和無(wú)DMD家族史的單個(gè)胚胎細(xì)胞,行DMD基因外顯子17、19、44、45、48的五重巢式PCR,分析擴(kuò)增成功率、假陽(yáng)性率和假陰性率。
In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay.
在對(duì)2份PPD檢測(cè)陰性而巢式PCR檢測(cè)陽(yáng)性的臨床樣本的檢測(cè)結(jié)果也為陽(yáng)性。