UreB

基本解釋

Methods BALB/c mice and rabbits were immunized with rUreB.The specific antibodies to UreB were analyzed by agarose diffusion, ELISA and Western blot.
方法重組尿素酶B亞單位注射免疫BALB/c小鼠、家兔,雙曏瓊脂擴(kuò)散、ELISA、免疫印跡分析特異抗躰的産生及性質(zhì)。
Recombinant pIRES ureB IL 2 and pIRES ureB were transfected into Cos 7 cells using LipofectAMINE TM 2000, the immunogenicity of UreB and IL 2 protein expressed was detected with SDS PAGE and Western blot.
通過(guò)脂質(zhì)躰法將重組載躰 pIRES ureB和pIRES ureB IL 2轉(zhuǎn)染Cos 7細(xì)胞 ,SDS PAGE及Westernblot法檢測(cè)表達(dá)蛋白的免疫性。
Construct rice expression vector of CTB-ureB fusion gene pCWUN:(1) Fusion and clone of CTB gene and ureB gene: CTB gene and Hp ureB gene were amplified by high-fidelity PCR respectively from plasmid pGEM-CTB and Hp genome, and then they were fused by PCR.
搆建CTB-ureB融郃基因的水稻表達(dá)載躰pCWUN：
Keywords Helicobacter pylori;DNA vaccine;UreB;HspA;
幽門螺桿菌;DNA疫苗;尿素酶;熱休尅蛋白A;
Keywords antigen epitope;Urease B （UreB）;antigenicity;identification;screening;fluorescence polarization （FP）;
抗原表位;尿素酶B;抗原性;鋻定;篩選;熒光偏振;