Lyss

基本解釋

Methods The gene encoding wide-type p53 was isolated,using RT-PCR from A549 cell line and cloned into pTATHA and pET32a prokaryotic expression vectors. Recombinant plasmids were E. coli BL21(DE3)LysS,then the transformed cells were induced with IPTG.
方法:利用RT-PCR方法從A549細(xì)胞系中分離野生型p53基因,將該基因分別克隆入pTATHA和pET32 a原核表達(dá)載體,在大腸桿菌BL21(DE3)LysS內(nèi)誘導(dǎo)表達(dá)并進(jìn)行純化。
The GST-ORF5 fusion protein has been expressed in Escherichia coli BL21 (DE3) LysS cells and anti-GST-ORF5 antiserum has been prepared. Characterization of ORF5product needs to be carried out further.
構(gòu)建了ORF5與pGEX-4T-2的GST融合表達(dá)載體,在大腸桿菌BL21中得到了表達(dá),并制備了兔多克隆抗體。
coli BL21(DE3)LysS and expressed with the induction of IPTG. The expression of the fusion proteins was analyzed by SDS-PAGE and Western blot.
在大腸桿菌BL21(DE3)LysS內(nèi)，以IPTG誘導(dǎo)融合蛋白表達(dá)，表達(dá)產(chǎn)物用SDS-PAGE及Western blot分析鑒定。